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Gut Microorganisms May Help Prevent Onset of Diabetes

Photo courtesy of iStockphoto, Silvia JansenYale researchers have found new supporting evidence to the so-called "hygiene hypothesis" - the theory that a lack of exposure to parasites, bacteria and viruses in the developed world may lead to increased risk of diseases like allergies, asthma, and other disorders of the immune system.In an animal study published in Nature, they found that exposure to certain non-pathogenic gut microorganisms helped prevent the onset of Type I diabetes in non-obese diabetic mice. From the report:Non-obese diabetic (NOD) mice develop the disease at different rates after natural breeding, depending upon the environment where they are kept. Previous research has shown that NOD mice exposed to killed (i.e., non-active) strains of tuberculosis or other disease-causing bacteria are protected against the development of Type I diabetes. This suggests that the rapid "innate" immune response that normally protects us from infections can influence the onset of Type 1 diabetes.In the Nature paper, teams led by Li Wen at Yale and Alexander V. Chervonsky at the University of Chicago showed that NOD mice deficient in innate immunity were protected from diabetes in normal conditions. However, if they were raised in a germ-free environment, lacking "friendly" gut bacteria, the mice developed severe diabetes. NOD mice exposed to harmless bacteria normally found in the human intestine were significantly less likely to develop diabetes, they reported.See full article.
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LifeSource Cryobank: Only Company in U.S. to Extract and Store Stem Cells Directly from Bone Marrow

LifeSource Cryobank: Only Company in U.S. to Extract and Store Stem Cells Directly from Bone Marrow

Photo courtesy of iStockphoto, Image# 1135046A privately-owned, FDA-registered, state of the art laboratory - LifeSource Cryobank, LLC - is committed to ensuring the safety and integrity of the stem cells it store in a totally secure environment. Located in Covington, Louisiana, LifeSource opened its doors in September 2007 and is the only company in the U.S. to extract and store stem cells directly from bone marrow.Utilizing an advanced medical technique, doctors at LifeSource Cryobank, LLC can extract, purify, expand and store a type of stem cells directly from bone marrow. Because these stem cells give rise to or participate in the formation of bone, muscle, cartilage, blood vessels and other tissues and organs, they are far superior to hematopoietic stem cells that can only regenerate new blood cells.This summer, Michael Drury became the first person in the United States to have his stem cells banked via the new extraction method. Developed by LifeSource Scientific Director Jose Minguell, Ph.D., and overseen by its Medical Director, Gabriel Lasala, M.D., Drury had stem cells aspirated directly from bone marrow in his hip. The stem cells were then isolated and multiplied before being stored in the cryopreservation facility.The researchers are also using these procedures to execute three Federal Drug Administration reviewed clinical trials to treat lower limb ischemia (blockage to the vessels of the legs), coronary ischemia (blockage to the vessels of the heart) and damage to heart muscle from heart attack. Over the last nine months, more than twenty people have undergone the patent-pending procedure as part of the trials.Find out more at LifeSource Cryobank, LLC.[Press release provided by Jill Willie of Gallinghouse & Associates.]See full article.
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Poly-D-Lysine Matrix for Growing Animal-Free Stem Cells

Poly-D-Lysine Matrix for Growing Animal-Free Stem Cells

Scientists have developed an alternative to animal-based materials such as Matrigel-coated plates for culturing the stem cells, which produce cells that are cells unsuitable for medical use. First, they identified a specific signaling pathway, called Rho-Rock, which the hESCs use during colony formation and which plays an important role in physical interactions between hESCs. When this pathway is blocked, researchers found that the normal colony formation of hESCs was considerably impaired, and that the hESCs maintained their pluripotency. In the study, sato links

's group extensively screened various types of scaffold materials in combination with Y27632, a chemical compound that blocks the Rho-Rock pathway, and found that the Matrigel coating could be replaced with "poly-D-lysine," a chemically synthesized ECM. The major advantages of poly-D-lysine over Matrigel are that poly-D-lysine is completely animal-free, easy to handle, and its quality is consistent."We found that the growth of the hESCs under this novel culture condition was almost identical to the growth of hESCs on Matrigel-coated culture plates, with no compromise in pluripotency," Sato said.
The group is now working on producing animal-free "induced pluripotent stem (iPS) cells" - pluripotent stem cells artificially derived from adult cells without using embryos.Photo: hESCs grown on poly-D-lysine-coated plate in defined culture media with Y27632. Photo credit: Sato lab, UC Riverside.See full article.
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Cardiosphere-Derived Stem Cells for Treatment of Heart Disease

Cardiosphere-Derived Stem Cells for Treatment of Heart Disease

Photo courtesy of iStockphoto, Aaliya LandholtDuring the recently held Southern California BioMedical Council (SoCalBio or SCBC) 10th Investor Conference, biotech firm Capricor Inc presented their Cardiosphere-derived Stem Cells or CDC technology, and the multi-billion dollar market potential for heart therapies using CDC. "CDCs are the perfect stem cells to treat heart attacks and chronic heart failure" said Foellmer [President and CEO]. " Our stem cells are easily accessible from the patient themselves, readily grown in large numbers, and have proven themselves extremely safe. Unlike embryonic stem cells, there are no ethical or moral quandaries. And unlike other adult stem cells, such as bone marrow stem cells, our CDCs actually regrow cardiac muscle and vasculature. In short, CDCs have great potential for patients with a recent heart attack or chronic heart failure and we eagerly look forward to our imminent clinical trials."Capricor Inc. is headquartered in Los Angeles, California, and specializes in discovering, developing, and commercializing biotherapeutics for the treatment of heart diseases. See their products currently under clinical trials.See full article.
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deCODE BreastCancer Genetic Diagnostic Test Launched

deCODE BreastCancer Genetic Diagnostic Test Launched

Photo courtesy of iStockphoto, Dawn PolanddeCoODE Genetics launched deCODE BreastCancer™, a new non-invasive tool for assessing risk of the common forms of breast cancer. The deCODE BreastCancer™ test measures seven widely replicated single-letter variations (SNPs) in the human genome that deCODE and others have linked to risk of breast cancer. These SNPs contribute to the incidence of an estimated 60 percent of all breast cancers. The test integrates data from discovery and replication studies published in major peer-reviewed journals and involving nearly 100,000 breast cancer patients and healthy volunteers from many populations, principally of European descent. deCODE and other organizations are conducting replication studies to validate these markers in populations of other continental ancestries.Note that this test measure genetic predisposition to breast cancer. It can identify the roughly 5 percent of women who are at a greater than 20 percent lifetime risk of the common forms of breast cancer (about twice the average risk in the general population), and the 1 percent of women whose lifetime risk is roughly 36 percent (about three-times average). These cases are different from the common forms of breast cancer comprising 95 percent of all breast cancers that result from the interplay of genetic as well as environmental and lifestyle factors.See full article.
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Wild Blueberries Have Most Antioxidant Than Common Fruits

Wild Blueberries Have Most Antioxidant Than Common Fruits

Photo courtesy of iStockphoto, Michal KoziarskiCornell University researchers have tested 25 fruits of its cellular antioxidant activity (CAA) and found that wild blueberries have greater antioxidant content than common fruits such as apples, bananas, red grapes and strawberries. Even superfruits such as noni, acai and goji did not measure up to wild blueberries.In addition to CAA, the researchers also measured total phenolic content, and oxygen radical absorbance capacity (ORAC) values. These are more typical 'in-the-fruit' readings but the researchers wanted to test the levels of antioxidants in fruits in a more biologically representative manner, hence their application of the CAA method which measures antioxidant activity 'in-the-cell'.Findings are published in the Journal of Agricultural and Food Chemistry.
See full article.
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Xylitol May Prevent Early Childhood Tooth Decay

Photo courtesy of iStockphoto, Craig Veltri In a recent clinical trial, researchers report a significant reduction of tooth decay in toddlers treated with the topical syrup xylitol, a naturally occurring non-cavity-causing sweetener.Researchers reported that nearly 76% of the children in the group who received xylitol were free of tooth decay by the end of the study, compared with 48% of the children in the group that did not receive the substance.Xylitol is FDA approved as a food additive, and is classified as safe. For the purpose reported here, it can be administered in the form of chewing gum, lozenges, or syrup.Source (pdf file)See full article.
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Photo courtesy of iStockphoto, Tammy Peluso Scientists have identified a compound called largazole, produced by cyanobacteria that grow on coral reefs off the coast of key largo links

, that may have potentials as an anti-cancer drug.Largazole, discovered and named by Luesch for its Florida location and structural features, seeks out a family of enzymes called histone deacetylase, or HDAC. Overactivity of certain HDACs has been associated with several cancers such as prostate and colon tumors, and inhibiting HDACs can activate tumor-suppressor genes that have been silenced in these cancers.Following promising results in initial laboratory tests, animal tests are currently being planned.See full article.
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Photo courtesy of iStockphoto, Doug Cannell New Zealand-based fruit science company HortResearch and Genesis Research and Development Corporation Limited have released over 130,000 kiwifruit gene sequences referred to as expressed sequence tags (ESTs). These are the active genes in the plant that govern such characteristics as flavour, colour, shape, vitamin content and aspects of fruit development such as ripening and storage life.The researchers say these data will be utilized in the development of new kiwifruit varieties links

through marker assisted selection (MAS).
In a MAS breeding programme, breeders use traditional crossing techniques to breed new varieties - which are then assessed for their commercial potential by searching their DNA for markers that indicate the presence of genes linked to desirable fruit traits. ESTs are essential in helping scientists identify the genes they're looking for."Our breeding programme with ZESPRI generates many thousands of seedlings every year. Without MAS, we would have to plant out each of those seedlings, wait years until they bear fruit and then assess which plants we wanted to commercialise or use for further breeding. With MAS, we can quickly "scan" the seedlings and find out right-away which ones are likely to have the type of fruit we want."

The genetic data have been published in BMC Genomics.SourceSee full article.
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Gpr41 Receptor Molecule in Intestines, Target for Anti-Obesity Drugs?

Gpr41 Receptor Molecule in Intestines, Target for Anti-Obesity Drugs?

Photo courtesy of iStockphoto, Sebastian KaulitzkiScientists have identified a receptor molecule present in the intestinal wall called Gpr4, that, when activated by wastes of gut bacteria, controls the movement of food through the intestine, thus regulating nutrient absorption and subsequently, gain weight.The researchers disrupted communication between the bacteria and the hosts in two ways: raising normal mice under germ-free conditions so they lacked the bacteria, and genetically engineering other mice to lack Gpr41 so they were unable to respond to the bacteria.In both cases, the mice weighed less and had a leaner build than their normal counterparts even though they all ate the same amount.The researchers also found that in mice without Gpr41, the intestines passed food more quickly. They hypothesized that one action of Gpr41 is to slow down the motion that propels food forward, so that more nutrients can be absorbed. Thus, if the receptor cannot be activated, food is expelled more quickly, and the animal gets less energy from it.

The researchers suggest that the Gpr41 receptor may be a likely target for drugs that can fight a certain kind of obesity by slowing down the absorption of energy from the gut.See full article.
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Silk Optics: Biodegradable and Biocompatible Optics

Silk Optics: Biodegradable and Biocompatible Optics

Photo courtesy of iStockphoto, Tor Lindqvist Biomedical engineers from Tuft's University have developed a novel silk-based optics that may be used in devices such as sensors which would combine sophisticated nanoscale optics with biological readout functions. In addition, silk optics offer further advantages in that they are biocompatible and biodegradable, and can be manufactured and stored at room temperatures without use of toxic chemicals.To form the devices, Tufts scientists boiled cocoons of the Bombyx mori silkworm in a water solution and extracted the glue-like sericin proteins. The purified silk protein solution was ultimately poured onto negative molds of ruled and holographic diffraction gratings with spacing as fine as 3600 grooves/mm. The cast silk solution was air dried to create solid fibroin silk films that were cured in water, dried and optically evaluated. A similar process was followed to create lenses, microlens arrays and holograms. Film thicknesses from 10 to 100 µm were characterized for transparency and optical quality.The Tufts team embedded three very different biological agents in the silk solution: a protein (hemoglobin), an enzyme (horseradish peroxidase) and an organic pH indicator (phenol red). In the hardened silk optical element, all three agents maintained their activity for long periods when simply stored on a shelf. "We have optical devices embedded with enzymes that are still active after almost a year of storage at room temperature. This is amazing given that the same enzyme becomes inactive if forgotten and left unrefrigerated for a few days," said Omenetto."

This research was published in the journal Biomacromolecules (you may download a copy here).
See full article.
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Tabebuia impetiginosa Extract May Help Reduce Obesity, Diabetes and Heart Disease Risks

Tabebuia impetiginosa Extract May Help Reduce Obesity, Diabetes and Heart Disease Risks

Using rats as animal models, scientists have been able to demonstrate that extracts derived from the Central and South American-native plant Tabebuia impetiginosa (also known as Pink Ipê or Pink Lapacho) can block dietary fat absorption, and thus reduce the risks of obesity, type 2 diabetes and coronary heart disease.Dr Roos and his team have shown that Tabebuia extract can reduce levels of triglycerides, a breakdown product of fat, in rats after they have been fed a fatty meal. "This result shows the extract may have a potential use in treating obesity," he observes. "However, as coronary heart disease and diabetes have also been shown to be associated with higher triglyceride levels after eating, we believe a food-supplement based on Tabebuia could reduce the incidence of these diseases as well. What is more, as obesity in developing countries is also on the increase, such extracts, taken as a capsule or added to food, may be a cheaper alternative for the rural population to pharmaceuticals."The data has been presented at the Society for Experimental Biology's Annual Meeting in Marseille last month and a paper is currently in press. The researchers say that futher studies will be conducted to identify the active compounds in the extracts.SourceSee full article.
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Insulin-Producing Cells from Embryonic Stem Cells

Photo courtesy of iStockphoto, Gary CavinessSingapore researchers have developed pure insulin-producing cells from mouse embryonic stem cells (ESCs). The resulting cells have the same sub-cellular structures as the insulin-producing cells naturally found in the pancreas, were highly effective in treating diabetes in the mouse model.Experiments by Dr Lim and Dr Li's group showed that when transplanted into diabetic mice with high blood glucose levels, the pure insulin-producing cells would cause the blood glucose levels of the animals to decrease. In addition, it was discovered that none of the diabetic mice involved in the transplants developed teratoma, a type of tumour often associated with ESCs, which could complicate their use in therapeutic treatment. Furthermore, the pure insulin-producing cells managed to retain their insulin-production and glucose-sensing capacity over time.These findings, published in two papers in the July 31 and August 8 online version of the journal Stem Cell Research, provide proof of principle that the same strategy could be applied to human ESCs to obtain similar pure insulin-producing cells.See full article.
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