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Phase 1 Trials Shows Plant-Produced Cancer Vaccine as Safe
Phase 1 Trials Shows Plant-Produced Cancer Vaccine as Safe
Photo courtesy of iStockphoto, Oleg Fedorkin Phase 1 trials of the the first human tests of a vaccine grown in tobacco plants proved that the vaccine against against follicular B-cell lymphoma were safe for patients. If further trials demonstrate its efficacy, this presents a novel method of producing vaccines quickly and cheaply. The researchers chose tobacco plants that were genetically modified to reproduce quantities of the vaccine. To make a tobacco plant churn out a human antibody, scientists isolate the antibody from the patient's tumor and put the antibody gene into a modified version of the tobacco mosaic virus. They infect a tobacco plant with the gene-carrying virus by scratching the virus on its leaves. The virus takes the gene into the plant's cells, which then churn out lots of antibody. After a few days, technicians snip off the plant's leaves, grind them up and purify the antibody. Only a few plants are needed to make enough vaccine for each patient."The new manufacturing system allows very rapid production of a vaccine," said Charles Arntzen, PhD, a professor of plant biology at the Arizona Biodesign Institute at Arizona State University, who was not involved in the research. "I think without the speed, it would be hard to convince a cancer patient to wait for a vaccine to be developed, rather than going on some other therapy."

The study is published in the advance online issue of the Proceedings of the National Academy of Science.
See full article.
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Research Services for Life Sciences, Launched by Symyx
The scientific R&D integration partner to companies in the life sciences, chemicals, energy, electronics and consumer products industries - Symyx Technologies, Inc. - has recently launched its new client-directed and collaborative Research Services for Life Sciences. These services allow clients to access Symyx's installed base of parallel experimentation and testing capabilities through packaged offerings for solubility studies, polymorph screening, salt selection, co-crystallization, API stability in liquid and solid formulations, excipientlinks compatibility, organic synthesis and process optimization.One of the first pharma company to sign an agreement with Symyx for Research Services for Life Sciences is Bristol-Myers Squibb. Research Services for Life Sciences, Launched by Symyx

Here are some ofthe benefits of Symyx's Research Services:
Poly-D-Lysine Matrix for Growing Animal-Free Stem Cells
Poly-D-Lysine Matrix for Growing Animal-Free Stem Cells
Scientists have developed an alternative to animal-based materials such as Matrigel-coated plates for culturing the stem cells, which produce cells that are cells unsuitable for medical use. First, they identified a specific signaling pathway, called Rho-Rock, which the hESCs use during colony formation and which plays an important role in physical interactions between hESCs. When this pathway is blocked, researchers found that the normal colony formation of hESCs was considerably impaired, and that the hESCs maintained their pluripotency. In the study, Sato's group extensively screened various types of scaffold materials in combination with Y27632, a chemical compound that blocks the Rho-Rock pathway, and found that the Matrigel coating could be replaced with "poly-D-lysine," a chemically synthesized ECM. The major advantages of poly-D-lysine over Matrigel are that poly-D-lysine is completely animal-free, easy to handle, and its quality is consistent."We found that the growth of the hESCs under this novel culture condition was almost identical to the growth of hESCs on Matrigel-coated culture plates, with no compromise in pluripotency," Sato said.
The group is now working on producing animal-free "induced pluripotent stem (iPS) cells" - pluripotent stem cells artificially derived from adult cells without using embryos.Photo: hESCs grown on poly-D-lysine-coated plate in defined culture media with Y27632. Photo credit: Sato lab, UC Riverside.See full article.
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Spinal Cord Stem Cells Identified
Spinal Cord Stem Cells Identified
Stem cells within the spinal cord that could differentiate into more healing cells and fewer scarring cells following an injury, have been identified. The researchers at MIT and the Karolinska Institute found that neural stem cells in the adult spinal cord are limited to a layer of cube- or column-shaped, cilia-covered cells called ependymal cells. These cells make up the thin membrane lining the inner-brain ventricles and the connecting central column of the spinal cord."We have been able to genetically mark this neural stem cell population and then follow their behavior," Meletis said. "We find that these cells proliferate upon spinal cord injury, migrate toward the injury site and differentiate over several months."
According to the scientists, if these cells can be regulated and genetically manipulated to to produce more myelinlinks and less scar tissue after a spinal cord injury, this may lead to a new, non-surgical treatment for debilitating spinal-cord injuries.Image: Coronal sections of injured adult spinal cord, anterior to posterior. The labelling shows recombined ependymal cells and their progeny (white) migrating out to the injury area in the dorsal funiculus, as a reponse to the injury (injury is in the image on the right hand corner). The mouse is a FoxJ1-CreER x R26R-LacZ reporter. Credit: Konstantinos MeletisSee full article.
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Seamless replication of data and results to an existing customer-managed Symyx database.
PostSecret: Fat And Pretend I’m Fine
This postcard from PostSecret showed up a couple of weeks ago. Since I grew up considering myself a fat child, I EXPECT people to make fun of me. In fact, I tend to make fun of myself to head off the insults. It must be difficult growing up skinny and becoming fat later in life. I [...]
Does Fast Food Make You Fat?
I came across this picture while I was researching gluttony the other day. It was labeled glutton.jpg. Wendy, Ronald McDonald and The KFC Colonel all have been rendered pudgy in front of a landscape of nutrition facts. The question remains, does fast food make you fat? When I lost my weight, I did it almost solely on [...]
Silk Optics: Biodegradable and Biocompatible Optics
Silk Optics: Biodegradable and Biocompatible Optics
Photo courtesy of iStockphoto, Tor Lindqvist Biomedical engineers from Tuft's University have developed a novel silk-based optics that may be used in devices such as sensors which would combine sophisticated nanoscale optics with biological readout functions. In addition, silk optics offer further advantages in that they are biocompatible and biodegradable, and can be manufactured and stored at room temperatures without use of toxic chemicals.To form the devices, Tufts scientists boiled cocoons of the Bombyx mori silkworm in a water solution and extracted the glue-like sericin proteins. The purified silk protein solution was ultimately poured onto negative molds of ruled and holographic diffraction gratings with spacing as fine as 3600 grooves/mm. The cast silk solution was air dried to create solid fibroin silk films that were cured in water, dried and optically evaluated. A similar process was followed to create lenses, microlens arrays and holograms. Film thicknesses from 10 to 100 µm were characterized for transparency and optical quality.The Tufts team embedded three very different biological agents in the silk solution: a protein (hemoglobin), an enzyme (horseradish peroxidase) and an organic pH indicator (phenol red). In the hardened silk optical element, all three agents maintained their activity for long periods when simply stored on a shelf. "We have optical devices embedded with enzymes that are still active after almost a year of storage at room temperature. This is amazing given that the same enzyme becomes inactive if forgotten and left unrefrigerated for a few days," said Omenetto."

This research was published in the journal Biomacromolecules (you may download a copy here).
See full article.
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Ask Laura: Nike ID
Laura, I went looking on the Nike site for some running shoes. Yes, I’m out running now. I’m working my way up using a podcast for interval running and my goal is to run a 5k in November. Anyways, I was looking at shoes and the Nike iD caught my eye. I [...]
I No Can Haz Peetsa
This LOL cat from I Can Has Cheezburger? is funny: It says: I no can haz peetsa, YOU no can haz peetsa For all of those not familiar with LOLspeak, he’s saying, “If I can’t have pizza, you can’t have pizza.” How many times have I felt like this? If I have to eat healthy, YOU [...]
Protein Transporter for Lutein and Zeaxanthin, Identified
Protein Transporter for Lutein and Zeaxanthin, Identified
© piddy77Scientists have identified a protein transporter for the compounds lutein and zeaxanthin, which are believed to protect against the development of age-related macular degeneration.These two nutrients are not made by the body and must be obtained through the diet. They are commonly found in green, leafy vegetables, such as kalelinks, spinach, broccoli, zucchini and peas, and in yellow or orange fruits and vegetables, such as carrots, papaya, squash and peaches.According to the study, the protein SR-B1, or scavenger receptor class B, type 1, plays a central role in transporting these nutrients from the bloodstream to cells in the eye.The research, published in the August issue of the Journal of Lipid Research, provides supporting evidence that lutein and zaxanthin may indeed be useful in preventing and/or treating age-related macular degeneration.
See full article.
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Enriched Boswellia serrata Extract Against Osteoarthritis
Enriched Boswellia serrata Extract Against Osteoarthritis
Photo courtesy of iStockphoto, Jeff Strickler In a study published in Arthritis Research and Therapy, researchers demonstrated the efficacy of 5-loxin, an extract of the 'Indian Frankincense' herb Boswellia serrata enriched with 30% 3-O-acetyl-11-keto-beta-boswellic acid (AKBA) in reducing the symptoms of osteoarthritis. "The high incidence of adverse affects associated with currently available medications has created great interest in the search for an effective and safe alternative treatment". The extract the authors used was enriched with 30% AKBA (3-O-acetyl-11-keto-beta-boswellic acid), which is thought to be the most active ingredient in the plant. Raychaudhuri said, "AKBA has anti-inflammatory properties, and we have shown that B. serrata enriched with AKBA can be an effective treatment for osteoarthritislinks of the knee". This is a proprietary product developed by Laila Nutraceuticals.Researchers also add that the results indicate that the compound had no major adverse effects in osteoarthritis patients and is safe for human consumption and even for long-term use.See full article.
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Sponsored Post: rVita, An Online Resource on Alternative Medicine - 05 May 2008




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Quote of the Month: August 2008
If you are one of the many people who have bought a Starling Fitness Yearly Journal, then you know that the quote of the month is about getting stronger. You can see the quote here: It is amazing how much crisper the general experience of life becomes when your body is given a chance [...]


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