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Assessing Calorie Restriction Effects
Ouroboros casts a skeptical eye on research I mentioned a little while ago at Fight Aging!: culturing cells using blood serum from calorie restriction (CR) and alternate day fasting (ADF) volunteers before and after the program to examine the differences. "we used sera collected from those studies to culture human hepatoma cells and assessed the effects on growth, stress resistance and gene expression. ... I was concerned by the lack of phenotypic benefits in cells treated with CR sera, as compared to cells treated with ADF sera: The CR-treated cells exhibited no increase in heat-shock resistance, no decrease in cell proliferation, and no correlation of Sirt1 expression to reduced triglyceride levels. In interpreting this finding, the authors suggest that the increased effects seen in the ADF participant serum could be due to the 'short, regular intervals of complete caloric deprivation,' which might provide a more potent serum profile capable of producing the observed in vitro changes. ... Alternatively, I think that these data could be a result of the cell type used in this assay (a tumor line); it would be interesting to see this experiment repeated on primary cells or a non-cancerous transformed cell line."

View the Article Under Discussion: http://ouroboros.wordpress.com/2008/10/02/feast-and-famine-in-vitro-techniques-to-assess-longevity-diets/
Read More Longevity Meme Commentary: http://www.longevitymeme.org/news/
Weight Loss Over 40 Plus Daily Record.....Week 11
Weight Loss Over 40 Plus Daily Record.....Week 11

Week 11: 70 pounds lost to date. My weight loss has slowed down. I think because my body is getting used to the workout program. I will change it up this week by adding strength training. The cardio (spin bike) has done a lot for my conditioning and endurance. I can not express enough how a very good pharmaceutical grade supplement helps.....True Essentials. I know at 800 calories a day without them I could not workout as hard as I do and get the results I do.


Walking 5 miles this weekweigh in: 249 up 2.5 pounds but losing inches and gaining muscle.

Please if I sound like a broken record forgive, but this has been the best thing I have every done for me. My quality of life has improved by a 100%, I am able to do more things now, than before I started. Every day is a new adventure and I am not sitting on my fat ass waiting to die. So please start a program for yourself today. If you would return next week to follow my wonderful journey.


Spin Bike 49 mins 4 days (19-19.5 miles a day)
Tai Chi... every day
Gazelle is still a 30 min workout
Gazelle 30 min workout other every day
Stop smoking
Spin Bike 49 mins (19.5-20 miles) every other day
Gazelle 30 min workout other every day
Gazelle 30 min workout... twice
An XM
Tai Chi for balance and flexibility.



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